Trim32 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN318203LP

Trim32 - mouse gene knockout kit via CRISPR, HDR mediated

Functional Cassette: GFP-puro Luciferase-Puro RFP-BSD mBFP-Neo



HDR-mediated knockout kit validation

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Specifications

Product Data
Format 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA Luciferase-Puro
Symbol Trim32
Locus ID 69807
Components

KN318203G1, Trim32 gRNA vector 1 in pCas-Guide CRISPR vector

KN318203G2, Trim32 gRNA vector 2 in pCas-Guide CRISPR vector

KN318203LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001161782, NM_053084
UniProt ID Q8CH72
Synonyms 3f3; 1810045E12Rik; BBS11; Zfp117
Summary Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin). May ubiquitinate BBS2 (By similarity). Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha.[UniProtKB/Swiss-Prot Function]

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