Stat3 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN316845RB
Stat3 - mouse gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro Luciferase-Puro mBFP-Neo
HDR-mediated knockout kit validation
Product Images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | Stat3 |
| Locus ID | 20848 |
| Components | KN316845G1, Stat3 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GTACAGCGACAGCTTCCCCA KN316845G2, Stat3 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GCAGCTGGACACACGCTACC KN316845RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. Homologous arm and RFP-BSD sequences: pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet CCATATGGGT GCTGGGAATT GAACTTGGTC CTCTGCAAGA GCAACAAGTG CATTAAACTA CTGAACCAGC TCTCCAGCAC ATGTGCTCTC TCTCCCCCTG TGTATGTGTG GGAATGGAGG TGGTGGCGAG GGTTTAGGGC TCAGGCTGGC CTTGACCTGT CTGTCATGAT ATCATAGGAG TTCTGGGATT ACAGGCATGA GCCGCTGTGC CTGGCTTCAA AGTAGATTCT AGAACTTAAA ATCACACCTA ATTGTAACAG AAGAAATTGC GTGGCAGCCT TGAATGGCAG CTCATGCCTG AAATTCAAGC ACTTAGGAGG CAGAGGTAGG AAATAACAAG GCTAGCCTGA GACCCTGTTT TAGAAAATCA AGGACCTGGA GACTAGCTCA GTGGTGGAGA ACTTGTATAG TGTTTGATCC CCAGCATCAG GAGTGGGCAG AGCTGTGGTG GTAGGATTTG TGAATGCAAA CACGTATTAT TCAAAGGACT ACAAAAAACA TACAAGCTCC TTTTCAGAGT CACCAGGGTA TCCCCTATGA GTACTTGTGG TGACCACATA TATATTTTGG CTTACAGTCG AGACCCCTGA CTGCAGCAGG AGACTGGTGA GTCCTTCTTG AAAACTCCCT GACTTGGGGG GTTTAGCATT GAGTCAGGAC ACAACCTGTT CTAGGTTCAC ACCTTACTAA CCCTTTGATC AGACATGTGA GGGCAGGTGC GGGGGAAGGA TTGGGAGAAC AGAGGTAACT TAGTCTCTTA ACGCATCTGT TCACAGTGCA TATGACAGCG GCCTCTTTGC ACTTTGAAAC AGCTTGCTGG TAGATGTTTT GGAGTCAGGT TTGAGTCTCA CTTACTTGGT GAGATCTCTG CACATTTTGA GTCTAGAACA GTGAGATACA GATGTTTTTC CTTTTTAAAT CTAAGAATTC TATGTCAGGT AGAAATTGGG GTCTACCACA CACGTGGCTT CCCCTGGTAA AGAGGAGGCA TGGGATGAGG AGGGAACTAG AGGCATAGAG GAAATGCCAG CTGTTTACCT CACTTTAGTG TATTCAGGCC CGTGTGCTAG TTCTGGCCTA AGTCAGCCCT ATCTGCGTGT GACCTACTTT TTCCAGAAGA CATACCTGAT TTGGTGATGG GCCAAAGAGG TGTGGATCAC ACCTCAGCCT GGCATTGCTC ATTAAGCTAA GAAGCACTTAGE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_011486, NM_213659, NM_213660 |
| UniProt ID | P42227 |
| Synonyms | 1110034C02Rik; Aprf; AW109958 |
| Summary | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN316845 | Stat3 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN316845BN | Stat3 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN316845LP | Stat3 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN516845 | Stat3 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA204124 | Stat3 CRISPRa kit - CRISPR gene activation of mouse signal transducer and activator of transcription 3 | 1 657,00 EUR |