Sirt6 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN315806BN
Sirt6 - mouse gene knockout kit via CRISPR, HDR mediated
HDR-mediated knockout kit validation
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Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control |
| Donor DNA | mBFP-Neo |
| Symbol | Sirt6 |
| Locus ID | 50721 |
| Components | KN315806G1, Sirt6 gRNA vector 1 in pCas-Guide CRISPR vector KN315806G2, Sirt6 gRNA vector 2 in pCas-Guide CRISPR vector KN315806BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001163430, NM_181586 |
| UniProt ID | P59941 |
| Synonyms | 2810449N18Rik; AI043036; Sir2l6 |
| Summary | NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate (By similarity). Acts as a corepressor of the transcription factor Hif1a to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. Regulates the production of TNF protein. Has a role in the regulation of life span in male mice, but not in female mice.[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN315806 | Sirt6 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN315806LP | Sirt6 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN315806RB | Sirt6 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN515806 | Sirt6 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA205336 | Sirt6 CRISPRa kit - CRISPR gene activation of mouse sirtuin 6 | 1 657,00 EUR |