Padi4 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN312748LP
Padi4 - mouse gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro RFP-BSD mBFP-Neo
HDR-mediated knockout kit validation
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Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
| Symbol | Padi4 |
| Locus ID | 18602 |
| Components | KN312748G1, Padi4 gRNA vector 1 in pCas-Guide CRISPR vector KN312748G2, Padi4 gRNA vector 2 in pCas-Guide CRISPR vector KN312748LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_011061 |
| UniProt ID | Q9Z183 |
| Synonyms | Pad4; Pdi4 |
| Summary | Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN312748 | Padi4 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN312748BN | Padi4 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN312748RB | Padi4 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN512748 | Padi4 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA203161 | Padi4 CRISPRa kit - CRISPR gene activation of mouse peptidyl arginine deiminase, type IV | 1 657,00 EUR |