Ngf Mouse Gene Knockout Kit (CRISPR)
CAT#: KN310963RB
Ngf - mouse gene knockout kit via CRISPR, HDR mediated
HDR-mediated knockout kit validation
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Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | Ngf |
| Locus ID | 18049 |
| Components | KN310963G1, Ngf gRNA vector 1 in pCas-Guide CRISPR vector KN310963G2, Ngf gRNA vector 2 in pCas-Guide CRISPR vector KN310963RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001112698, NM_013609 |
| UniProt ID | P01139 |
| Synonyms | Ngfb |
| Summary | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed:20036257). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (PubMed:22649032). The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse (PubMed:22155786). In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (PubMed:20036257). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (By similarity). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer (PubMed:22649032, PubMed:26144237). The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (PubMed:22649032).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN310963 | Ngf - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN310963BN | Ngf - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN310963LP | Ngf - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN510963 | Ngf - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA202896 | Ngf CRISPRa kit - CRISPR gene activation of mouse nerve growth factor | 1 657,00 EUR |