Klf15 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN308828
Klf15 - mouse gene knockout kit via CRISPR, HDR mediated
Functional Cassette: Luciferase-Puro RFP-BSD mBFP-Neo
HDR-mediated knockout kit validation
Product Images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control |
| Donor DNA | GFP-puro |
| Symbol | Klf15 |
| Locus ID | 66277 |
| Components | KN308828G1, Klf15 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GACGAGAAGGTCTCGTCCAC KN308828G2, Klf15 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CATGTGGTATGGCTGCCGGC KN308828D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences: pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CAGCCCAGAC AGATGTGTGT CCCATCCCTT CCAGTCCTGG GGACACCATG CTCACATCCA CAGATGTTCA TTTTTGAACA TCTAAAGTCA ACATCGAACA AGCAGCCTGT TTTTATTTGC TTAATCTGGA AACCCTACAG AAGCCCCAAT ACCTTGAGCT CAGAATGCAA ATGGCCTTTT CCTTTCTCAC CCCCTCTCTG ACTCAAGGTA GGAAGCAGAG TCCAGGGGCT GGCGACTTTC AGGGCCTCCC CTCTCTGGCT CCCTCTCTCC TCGTGGCCTC AGCTGTTCGC GGACAAGAGC GTTCCTTGTC TTATTCTTTT CCAGGATCAT TCTGGAAAAT GTTTGAAACC AGAAGTGCCC TGGATTGTGC AACGGGCCTG GAGAGGGAGG GCTCTTTTGC AAATAGGGTG ATGCAACTTT TGAAAACATA CTCGCCGGCC CCATTCTCCC TTGGGATGGA AGCAGGATCT GGACATTTGG CCACAGGAGG CCTTGGGCAG GCAGCCGAGG GTGAGGGCTG CAAGGCACTG GTAAGCAGTC CTTCTAACCT GGCCTCCCTT GTTCCCTCTC TGCAGTCACC ACAGGCTCGG CCAGGCCAGC CCCCTGCTCT TGTGCCAGCC CTGACTCGCA AGCCTTCTGT TCCTGCTACA GTGCGGGTCC AGGCCCTGAG GCCCAGGGCA GCATCTTGGA TTTCCTCCTG TCCCGGGCCA CACTGGGCAG TGGTGGTGGC AGTGGAGGTA TTGGAGATAG CAGTGGCCCT GTGACCTGGG GATCATGGAG GAGAGCCTCT GTGCCTGTGA AGGAGGAACA TTTCTGCTTC CCTGAATTTC TGTCAGGGGA CACTGATGAC GTCTCCAGGC CCTTCCAGCC TACCCTGGAG GAGATTGAAG AATTCCTGGA AGAGAACATG GAGGCTGAGG TCAAGGAGGC CCCAGAGAAC GGTAGCAGGG ACCTGGAGAC CTGTAGCCAG CTCTCAGCTG GGTCACACCG GAGCCACCTT CATCCAGAGT CTGCTGGGAG AGAGCGCTGT ACCCCACCAC CAGGTGGCAC GAGTGGGGGT GGTGCCCAAA GTGCAGGTGA GGGGCCAGCA CATGATGGCC CCGTGCCGGT GCTACTGCAG ATCCAGCCTG TTGCTGTGAA GCAGGAGGCA GGTACAGGGC CAGCCTCCCC AGGGCAGGCC CCAGAGAGCG TCAAGGTCGCGE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_023184, NM_001355668 |
| UniProt ID | Q9EPW2 |
| Synonyms | 1810013I09Rik; AV048136; AW494632; CKLF; KKLF |
| Summary | Transcriptional regulator that binds to the GA element of the CLCNKA promoter (By similarity). Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart. Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. Is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A, GATA4 and MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation (By similarity). Involved in podocyte differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
|
| SDS |
Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN308828BN | Klf15 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN308828LP | Klf15 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN308828RB | Klf15 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN508828 | Klf15 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA206684 | Klf15 CRISPRa kit - CRISPR gene activation of mouse Kruppel-like factor 15 | 1 657,00 EUR |