Dpp4 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN304781LP
Dpp4 - mouse gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro RFP-BSD mBFP-Neo
HDR-mediated knockout kit validation
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Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
| Symbol | Dpp4 |
| Locus ID | 13482 |
| Components | KN304781G1, Dpp4 gRNA vector 1 in pCas-Guide CRISPR vector KN304781G2, Dpp4 gRNA vector 2 in pCas-Guide CRISPR vector KN304781LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. Homologous arm and Luciferase-Puro sequences: pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet TTACTTTTTT GCGGGGGGAG GGACTCCGGC AAAGCAAGTG AACATTCCCC AGGGAGGATT AATACAGATA TCCAACCTGA AGTGTCATTC AAAAGGGAAC AATCACCTTT TCTCTTTCTT TGCCAGCCAG ATAACATGGG ACACCCAACC CCTTCTCTCA GACTGACAGA CCTCTCAGGG AAGGGGACAA GAGAACTGGC AGCTAGTTTC TGCAGTGAGC CGTGAATCTG CCAAACTCCA TGCCTGCTTG AACCTCAGCT GCCTCGATTA TCCACGCAAG GGAGCAACAG TTAAACCTCA ATGTGCAATG TCTCTTTTAG CAAAGGGGGT TCGCTGTTCC CCTTATCCCT CACTCATGGG GGCCCTGCGT GCTACTTCCT GGCTCGCCTA GTGGTCCTAC CGCGCTGGGT CGGCTGCCCC GCGCTCACAC TGTGCACCGA AGCCCGCCTT GCGCTAACTA ATGTTTAACT CAGGCCGAAA CTTGGCGGCG AGCTCAGGGT GACTGCGTGC AGAGCAGCCG CGCAGGACGT CCGTCTCTGC GCGCAGTGAC TTCTGCCTGC GCTCAAGCTT CAGAGTTCAG TTTCAAGGAG CCGCCCGACC GCGGAAGCCA CGGGGCCACC CTCACAGGTG AAGGCACGTC CGTCCCGGGA ACTGGCTTAG ATCTCTGGCG TGTCCTACTG GGACCCAGGC ACTGAGCCTT GGAAGTCGGG GACGGTCACC CGTGGAACGC ACGCGGCACT CTCATTTGGC ATCAGACGGG TGGGTGGGAG GAGAGAAGCC AACAAGATCA TAAGATCATG CTCAGGGCCA AAATTCAAGG GCTTCTGCCT GAGTGAACGC CAAACCCCTC AGGTCTACTA GTGCACCTGC AAACTCCTCT GCCTCTGAAG GGGCCTAAGT CCCAACCCTC TTCGCAGAGA GGAACAAAGA CTTCCCCTCT AATTGTTTGC AATCTGACCT GCTTGCAGAC ACCGTGGAAG GTTCTTCTGG GACTGCTTGG TGTCGCTGCG CTTGTCACCA TCATCACCGT GCCAATAGTT CTGCTGAGCA AAGGTAGAAC CTTGCAACAT CTTAAAAAGA GAGGGTGGTT GGAGACGGGG TCAGGCGGCT CTCAAAGCGA GCTCAGTTTA TTGGGGGGCC TTGACCCCTG TGCTGGGTTA TGGCTGAAGA GACTGGTAAG TGTCTCTAAA TGTATTTGCTGE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001159543, NM_010074 |
| UniProt ID | P28843 |
| Synonyms | Cd26; Dpp-4; THAM |
| Summary | Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN304781 | Dpp4 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN304781BN | Dpp4 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN304781RB | Dpp4 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN504781 | Dpp4 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA201121 | Dpp4 CRISPRa kit - CRISPR gene activation of mouse dipeptidylpeptidase 4 | 1 657,00 EUR |