Depdc5 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN304504BN

Depdc5 - mouse gene knockout kit via CRISPR, HDR mediated



HDR-mediated knockout kit validation

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Specifications

Product Data
Format 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Donor DNA mBFP-Neo
Symbol Depdc5
Locus ID 277854
Components

KN304504G1, Depdc5 gRNA vector 1 in pCas-Guide CRISPR vector

KN304504G2, Depdc5 gRNA vector 2 in pCas-Guide CRISPR vector

KN304504BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001025426, NM_001170567, NM_177786, NM_001360014
UniProt ID P61460
Synonyms AV016528
Summary As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function]

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