Ddit3 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN304365LP
Ddit3 - mouse gene knockout kit via CRISPR, HDR mediated
HDR-mediated knockout kit validation
Product Images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
| Symbol | Ddit3 |
| Locus ID | 13198 |
| Components | KN304365G1, Ddit3 gRNA vector 1 in pCas-Guide CRISPR vector KN304365G2, Ddit3 gRNA vector 2 in pCas-Guide CRISPR vector KN304365LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001290183, NM_007837 |
| UniProt ID | P35639 |
| Synonyms | chop; CHOP-10; CHOP10; gadd153 |
| Summary | Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response. Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling.[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN304365 | Ddit3 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN304365BN | Ddit3 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN304365RB | Ddit3 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN504365 | Ddit3 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA201042 | Ddit3 CRISPRa kit - CRISPR gene activation of mouse DNA-damage inducible transcript 3 | 1 657,00 EUR |