Dclk1 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN304323LP
Dclk1 - mouse gene knockout kit via CRISPR, HDR mediated
HDR-mediated knockout kit validation
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Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
| Symbol | Dclk1 |
| Locus ID | 13175 |
| Components | KN304323G1, Dclk1 gRNA vector 1 in pCas-Guide CRISPR vector KN304323G2, Dclk1 gRNA vector 2 in pCas-Guide CRISPR vector KN304323LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001111051, NM_001111052, NM_001111053, NM_001195538, NM_001195539, NM_001195540, NM_019978 |
| UniProt ID | Q9JLM8 |
| Synonyms | 1700113D08Rik; 2810480F11Rik; AI836758; Click-I; Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369 |
| Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the biological validity of some variants has not been determined. These variants encode different isoforms, which are differentially expressed and have different kinase activities. [provided by RefSeq, Sep 2010] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN304323 | Dclk1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN304323BN | Dclk1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN304323RB | Dclk1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN504323 | Dclk1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA201027 | Dclk1 CRISPRa kit - CRISPR gene activation of mouse doublecortin-like kinase 1 | 1 657,00 EUR |