Ctla4 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN303962LP

Ctla4 - mouse gene knockout kit via CRISPR, HDR mediated



HDR-mediated knockout kit validation

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Specifications

Product Data
Format 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA Luciferase-Puro
Symbol Ctla4
Locus ID 12477
Components

KN303962G1, Ctla4 gRNA vector 1 in pCas-Guide CRISPR vector

KN303962G2, Ctla4 gRNA vector 2 in pCas-Guide CRISPR vector

KN303962LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001281976, NM_009843
UniProt ID P09793
Synonyms Cd152; Ctla-4; Ly-56
Summary This gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]

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