C1galt1c1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN302341RB

C1galt1c1 - mouse gene knockout kit via CRISPR, HDR mediated

Functional Cassette: GFP-puro Luciferase-Puro RFP-BSD mBFP-Neo



HDR-mediated knockout kit validation

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Specifications

Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA RFP-BSD
Symbol C1galt1c1
Locus ID 59048
Components

KN302341G1, C1galt1c1 gRNA vector 1 in pCas-Guide CRISPR vector

KN302341G2, C1galt1c1 gRNA vector 2 in pCas-Guide CRISPR vector

KN302341RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_021550
UniProt ID Q9JMG2
Synonyms 1500002I11Rik; AB030184; C1GalT2; C81205; Cosmc
Summary Probable chaperone required for the generation of 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Probably acts as a specific molecular chaperone assisting the folding/stability of core 1 beta-3-galactosyltransferase (C1GALT1) (By similarity).[UniProtKB/Swiss-Prot Function]

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