Brca2 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN302249RB
Brca2 - mouse gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro Luciferase-Puro mBFP-Neo
HDR-mediated knockout kit validation
Product Images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | Brca2 |
| Locus ID | 12190 |
| Components | KN302249G1, Brca2 gRNA vector 1 in pCas-Guide CRISPR vector KN302249G2, Brca2 gRNA vector 2 in pCas-Guide CRISPR vector KN302249RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001081001, NM_009765 |
| UniProt ID | P97929 |
| Synonyms | Fancd1; RAB163 |
| Summary | Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability, independently of its known role in homologous recombination (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN302249 | Brca2 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN302249BN | Brca2 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN302249LP | Brca2 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN502249 | Brca2 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA200439 | Brca2 CRISPRa kit - CRISPR gene activation of mouse breast cancer 2, early onset | 1 657,00 EUR |