Aifm1 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN301038RB
Aifm1 - mouse gene knockout kit via CRISPR, HDR mediated
HDR-mediated knockout kit validation
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Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | Aifm1 |
| Locus ID | 26926 |
| Components | KN301038G1, Aifm1 gRNA vector 1 in pCas-Guide CRISPR vector KN301038G2, Aifm1 gRNA vector 2 in pCas-Guide CRISPR vector KN301038RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001290364, NM_012019 |
| UniProt ID | Q9Z0X1 |
| Synonyms | AIF; AIFsh2; Hq; Pdcd8 |
| Summary | Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN301038 | Aifm1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN301038BN | Aifm1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN301038LP | Aifm1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN501038 | Aifm1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA205043 | Aifm1 CRISPRa kit - CRISPR gene activation of mouse apoptosis-inducing factor, mitochondrion-associated 1 | 1 657,00 EUR |