NMNAT1 ELISA Kit (Mouse)

Cat# OKWB00261

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for NMNAT1 ELISA Kit (Mouse) (OKWB00261)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-105%86-104%95-103%88-104%
EDTA Plasma (n=5)84-101%85-100%86-94%82-98%
Heparin Plasma (n=5)80-93%86-98%86-97%80-98%
ELISA Kit PrincipleAviva Systems Biology Nmnat1 ELISA Kit (Mouse) (OKWB00261) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nmnat1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nmnat1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nmnat1 captured in the well.
ELISA Kit Range15.625-1000pg/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)90-10596
EDTA Plasma (n=5)85-10092
Heparin Plasma (n=5)85-10498
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Nmnat1 Microplate96 Wells (12 x 8 Well strips)
Nmnat1 Lyophilized Standard2 x 1 ng
100X Nmnat1 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity9.4 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolNmnat1
Gene Full Namenicotinamide nucleotide adenylyltransferase 1
Alias Symbols2610529L11Rik;5730441G13Rik;D4Cole;D4Cole1e;naMN adenylyltransferase 1;nicotinamide mononucleotide adenylyl transferase;nicotinamide mononucleotide adenylyltransferase 1;nicotinamide/nicotinate mononucleotide adenylyltransferase 1;nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1;nicotinate-nucleotide adenylyltransferase 1;nm;NMN adenylyltransferase 1;NMN/NaMN adenylyltransferase 1;nmnat.
NCBI Gene Id66454
Protein NameNicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
Description of TargetCatalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed:15381699). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (By similarity). Can use triazofurin monophosphate (TrMP) as substrate (By similarity). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (By similarity). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (By similarity). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (By similarity). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (By similarity). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (By similarity). Protects against axonal degeneration following mechanical or toxic insults (PubMed:15310905, PubMed:16914673). Delays axonal degeneration after axotomy. Results in a >10-fold increase in intact neurites 72 hours after injury (PubMed:16914673).
Uniprot IDQ9EPA7
Protein Accession #NP_597679
Nucleotide Accession #NM_133435

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