Specifications

Product Data
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse TNF alpha
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	15.6 pg/ml-1000 pg/ml
Sample Type	Cell culture supernates, serum and plasma (heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of mouse TNF alpha
Sensitivity	<1pg/ml
Reactivities	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti-mouse TNF alpha antibody | 1 Lyophilized recombinant mouse TNF alpha standard | 10ng/tube×2 Biotinylated anti-mouse TNF alpha antibody | 100μl (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Plate Sealers | 4 Wash Buffer (25x) | 20 ml
Background	Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
Gene Symbol	Tnf
Gene ID	21926