Specifications

Product Data
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse NGF/NGF beta
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	31.2pg/ml-2000pg/ml
Sample Type	Cell culture supernates and serum.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of mouse NGF
Sensitivity	<1pg/ml
Reactivities	Mouse
Cross Reactivity	There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- mouse NGF/NGF beta antibody 1 Lyophilized recombinant mouse NGF/NGF beta standard 10ng/tubex2 Biotinylated anti- mouse NGF/NGF beta antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.
Gene Symbol	Ngf
Gene ID	18049
Standard Curve	Representative standard curve for Mouse NGF/NGF beta PicoKine ELISA Kit