Mouse IL-17A ELISA Kit (96-well)

CAT#: EA100158

Mouse IL-17A ELISA Kit (96-well)

Size: 1 x 48 wells 1 x 96 wells


Product Images

Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hour
Signal Colorimetric
Curve Range 7.8 - 500 pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Mouse IL-17A Ligand
Sensitivity 4pg/mL
Reactivities Mouse
Interference No significant interference observed with available related molecules.
Components
  • Aluminium pouches with a Microwell Plate coated with antibody to mouse IL-17 (8x12)
  • 2 vials mouse IL-17 Standard lyophilized,500 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse IL-17 antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
Background

Mouse Interleukin 17 (IL-17; also known as IL-17A and CTLA-8) is a 21 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines containing a cysteine-knot fold . Its sequence was originally isolated from an activated hybridoma created from the fusion of a mouse cytotoxic and rat T cell lymphoma cell line . It is synthesized as a 158 amino acid (aa) precursor that contains a 25 aa signal sequence and a 15 kDa, 133 aa mature segment . In both mouse and human, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 35-38 kDa homodimer,and a 45-48 kDa heterodimer with IL-17F . Mature mouse IL-17A is 61% and 89% aa identical to human and rat IL-17A, respectively . While rodent and human mature sequences show modest aa sequence identity, human IL-17 is active on both mouse and rat cells . Cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27-.. T cells, CD1+NK1.1- iNKT cells and CD3-CD4+ LTi-like cells(3, 5, 6, 10-12).

A high affinity receptor for mouse IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio . IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily . IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30% aa identity with IL-17RA . Both receptors are needed for IL-17A and IL-17A/F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA .

IL-17 is best known for its participation in the recruitment and survival of neutrophils . Its induction was initially described to be the result of antigen stimulation of dentritic cells, resulting in IL-23 secretion. In a TCR-independent event, IL-23 induces T cell production of IL-17. Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and SCF (membrane form), an effect that increases neutrophil differentiation and activation. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating neutrophil numbers . In the tissues, IL-17 seems to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-., IL-1. and IL-6 production .TNF-. and IL-1. then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 . IL-17 further contributes to neutrophil influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability . IL-17 effects are not limited to neutrophils. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption .

Gene Symbol Il17a
Gene ID 16171
Standard Curve
Representative standard curve for IL-17 ELISA. IL-17 was diluted in serial two-fold steps in Sample Diluent.

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