Mouse CTLA4 ELISA Kit

CAT#: EA100458

Mouse CTLA4 ELISA Kit


Product Images

Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse CTLA4
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse CTLA4
Sensitivity <10pg/ml
Reactivities Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse CTLA4 antibody 1
  • Lyophilized recombinant mouse CTLA4 standard 10ng/tubex2
  • Biotinylated anti- mouse CTLA4 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background CTLA-4(Cytotoxic T-Lymphocyte Antigen 4), also known as CD152(Cluster of differentiation 152), is a protein receptor that down regulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.
Gene Symbol Ctla4
Gene ID 12477

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