Specifications

Product Data
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	5-100 ng/ml
Sample Type	Serum
Sample Volume	10 µl/well
Specificity	This kit is used for quantitative detection Cotinine (Mouse/Rat)
Sensitivity	5 ng/ml
Reactivities	Mouse, Rat
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Microwell coated with polyclonal Ab to Cotinine: 12x8x1 2. Standard Set (ready to use): 0.5 ml 3. Cotinine HRP Enzyme Conjugate (ready to use): 12 ml 4. TMB Substrate (ready to use): 12 ml 5. Stop Solution (ready to use): 12 ml 6. 20X Wash concentrate: 1 bottle: 25ml
Background	Exposure to tobacco smoke can be detected by measuring nicotine and its metabolites. Nicotine has a short half life and is not used as a marker for tobacco smoke exposure. Cotinine due to its longer half life has been used in research as a reliable marker for smoking status and smoking cessation studies. The Cotinine Direct ELISA Kit is designed for the detection Cotinine in serum and urine. It can also be adapted for other fluids.