Human Alpha A Crystallin/CRYAA antibody
Cat# ACA0401-100
Size : 100ul
Brand : NKMAX BIO
The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. This antibody is not shown cross-activity about Crystallin alpha B.
Lane 1.: Recombinant human Crystallin alpha A protein
Lane 2.: Recombinant human Crystallin alpha B protein
Lane 1.: Recombinant human Crystallin alpha A protein
Lane 2.: Recombinant human Crystallin alpha B protein
The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Crystallin alpha A Transfected 293T cell lysate
Lane 1.: 293T cell lysate
Lane 2.: Crystallin alpha A Transfected 293T cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of Crystallin alpha A in Balb/3T3 cells. The cell was stained with ACA0401 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.