Images
Western Blot (Tissue lysate)
Application

Western Blot (Tissue lysate)

Western blot analysis of Lane 1: mouse kidney tissue lysate; Lane 2: mouse brain tissue lysate; Lane 3: mouse liver tissue lysate.

Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot analysis of Lane 1: Jurkat cell lysate; Lane 2: HeLa cell lysate; Lane 3: K562 cell lysate; Lane 4: MCF7 cell lysate; Lane 5: A549 cell lysate; Lane 6: Raji cell lysate. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL.

Immunocytochemistry
Application

Immunocytochemistry

Immunocytochemistry analysis of HeLa cells. The cell was stained with PPM1A monoclonal antibody, clone p6c7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

  • Specification

    Product Description

    Mouse monoclonal antibody raised against partial recombinant PPM1A.

    Immunogen

    Recombinant protein corresponding to amino acids 1-382 of human PPM1A.

    Host

    Mouse

    Reactivity

    Human, Mouse

    Form

    Liquid

    Purification

    Protein G affinity chromatography

    Isotype

    IgG2b, kappa

    Recommend Usage

    ELISA
    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).

    Storage Instruction

    Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Tissue lysate)

    Western blot analysis of Lane 1: mouse kidney tissue lysate; Lane 2: mouse brain tissue lysate; Lane 3: mouse liver tissue lysate.

    Western Blot (Cell lysate)

    Western blot analysis of Lane 1: Jurkat cell lysate; Lane 2: HeLa cell lysate; Lane 3: K562 cell lysate; Lane 4: MCF7 cell lysate; Lane 5: A549 cell lysate; Lane 6: Raji cell lysate. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL.

    Immunocytochemistry

    Immunocytochemistry analysis of HeLa cells. The cell was stained with PPM1A monoclonal antibody, clone p6c7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

    Immunofluorescence

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — PPM1A

    Entrez GeneID

    5494

    GeneBank Accession#

    NM_177952

    Protein Accession#

    P35813

    Gene Name

    PPM1A

    Gene Alias

    FLJ42306, MGC9201, PP2C-ALPHA, PP2CA

    Gene Description

    protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform

    Omim ID

    606108

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq

    Other Designations

    protein phosphatase 1A|protein phosphatase 2C alpha isoform

  • Interactome
  • Pathway
  • Disease
  • Publication Reference