CNOT1 Human Gene Knockout Kit (CRISPR)
CAT#: KN206903RB
CNOT1 - human gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro Luciferase-Puro mBFP-Neo
HDR-mediated knockout kit validation
Product Images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | CNOT1 |
| Locus ID | 23019 |
| Components | KN206903G1, CNOT1 gRNA vector 1 in pCas-Guide CRISPR vector KN206903G2, CNOT1 gRNA vector 2 in pCas-Guide CRISPR vector KN206903RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001265612, NM_016284, NM_206999, NR_049763 |
| UniProt ID | A5YKK6 |
| Synonyms | AD-005; CDC39; NOT1; NOT1H |
| Summary | Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3' UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity.[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN206903 | CNOT1 - human gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN206903BN | CNOT1 - human gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN206903LP | CNOT1 - human gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN406903 | CNOT1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA107973 | CNOT1 CRISPRa kit - CRISPR gene activation of human CCR4-NOT transcription complex subunit 1 | 1 657,00 EUR |