CBFB Human Gene Knockout Kit (CRISPR)
CAT#: KN202111RB
CBFB - human gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro Luciferase-Puro mBFP-Neo
HDR-mediated knockout kit validation
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Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | CBFB |
| Locus ID | 865 |
| Components | KN202111G1, CBFB gRNA vector 1 in pCas-Guide CRISPR vector KN202111G2, CBFB gRNA vector 2 in pCas-Guide CRISPR vector KN202111RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001755, NM_022845, NM_001368710, NM_001368707, NM_001368708, NM_001368709 |
| UniProt ID | Q13951 |
| Synonyms | PEBP2B |
| Summary | The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN202111 | CBFB - human gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN202111BN | CBFB - human gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN202111LP | CBFB - human gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN402111 | CBFB - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA100609 | CBFB CRISPRa kit - CRISPR gene activation of human core-binding factor subunit beta | 1 657,00 EUR |