Total MMP-1 ELISA Kit (Human)

Cat# OKAG00170

Size : 1plate:12x8-WellMicrostrips

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric 450 nm
ELISA Kit Duration4.5 hours
ELISA Kit PrincipleThe Aviva Human MMP-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hMMP-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human MMP-1 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range157-10000 pg/ml
ELISA Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (6 ng)
Reconstitution and Storage2°C to 8°C
Sample TypeCell lysates, sera and plasma
Sensitivity157 pg/mL
SpecificityThe Human MMP-1 ELISA is capable of recognizing both recombinant and naturally produced Human MMP-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-12, MMP-13, MMP-16, TIMP-1, TIMP-2, TIMP-3, TIMP-4
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolMMP1
Gene Full Namematrix metallopeptidase 1
Alias SymbolsCLG;CLGN;fibroblast collagenase;interstitial collagenase;matrix metallopeptidase 1 (interstitial collagenase);matrix metalloprotease 1;Matrix metalloproteinase-1.
NCBI Gene Id4312
Protein NameP03956
Description of TargetInterstitial collagenase
Uniprot IDhttps://www.uniprot.org/uniprot/P03956
Protein Accession #NP_002412.1
Nucleotide Accession #NM_002421.3

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