NPY ELISA Kit (Human)

Cat# OKEH00652

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00652
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NPY ELISA Kit (Human) (OKEH00652) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPY has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPY is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NPY captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-NPY Microplate96 Wells (12 x 8 Well strips)
NPY Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated NPY Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity32 pg/mL
SpecificityNatural and recombinant Human Pro-neuropeptide Y
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNPY
Gene Full Nameneuropeptide Y
Alias Symbolsprepro-neuropeptide Y;pro-neuropeptide Y;PYY4.
NCBI Gene Id4852
Protein NameP01303
Description of TargetPro-neuropeptide Y
Uniprot IDhttps://www.uniprot.org/uniprot/P01303
Protein Accession #NP_000896.1
Nucleotide Accession #NM_000905.3
Protein Size (# AA)97
Molecular Weight10 kDa

You might also be interested by the following products:



Cat#
Description
Cond.
Price Bef. VAT
OKEH02558
 96Wells