Human IL-6 ELISA Kit (96-well)

CAT#: EA100030

Human IL-6 ELISA Kit (96-well)

Size: 1 x 48 wells 1 x 96 wells



Product Manual for 'EA100030'

Product Images

Specifications

Product Data
Format 48-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 3.9-250pg/ml
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Human IL-6 Ligand
Sensitivity 2pg/mL
Reactivities Human
Interference No significant interference observed with available related molecules.
Components Aluminium pouches with a Microwell Plate coated with antibody to human IL-6 (8 x 6)
2 vials human IL-6 Standard lyophilized, 1000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-6 antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
4 pieces Adhesive Films
package insert
Background

Interleukin 6(IL-6) is a multifunctional protein produced by lymphoid and non-lymphoid cells and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. For reviews on IL-6, see references 1-5.

The human IL-6 cDNA sequence predicts a protein of 212 amino acid (aa) residues in length with two potential N-glycosylation sites. The hydrophobic N-terminal 28 aa residue signal peptide is cleaved to produce a mature protein of 184 amino acids with four cysteine residues and a predicted molecular mass of 21 kDa . The mouse IL-6 cDNA sequence shows a homology of 42% at the aa level when compared with the human sequence . On the basis of sequence similarity and gene structural motif similarity, IL-6 can be grouped in a family of cytokines that also includes OSM, G-CSF, LIF, and CNTF. All of these cytokines are predicted to have a four helix bundle structure similar to that found for growth hormone, suggesting that they all evolved from a common ancestral gene .

The effects of IL-6 on different cells are numerous and varied. The effect on B cells is stimulation of differentiation and antibody secretion . IL-6 also affects T cells, acting as a co-stimulant with sub-optimal concentrations of PHA or Con A to stimulate IL-2 production and IL-2 receptor expression.

IL-6 exhibits growth factor activity for mature thymic or peripheral T-cells and reportedly enhances the differentiation of cytotoxic T-cells in the presence of IL-2 or IFN-. . IL-6 stimulates production of acute phase proteins by hepatocytes and has colony-stimulating activity on hematopoietic stem cells. IL-6 has growth factor activities and will stimulate the growth of myeloma/hybridoma/ plasmacytoma cells, EBV-transformed B cells, keratinocytes and mesangial cells .

Gene Symbol IL6
Gene ID 3569
Standard Curve
Representative standard curve for IL-6 ELISA. IL-6 was diluted in serial two-fold steps in Sample Diluent.