Human IL-33 ELISA Kit, 1 x 96-well (pre-coated)

Specifications

Product Data
Format	1 x 96-well (pre-coated)
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3h45min
Signal	Colorimetric
Curve Range	31.25-1000 pg/ml
Sample Type	Serum, Plasma and other body fluids, Cell culture supernatants, Buffered solutions
Sample Volume	100 µl (50µl for serum/plasma)
Specificity	Recognizes both natural and recombinant human IL-33
Sensitivity	12pg/ml
Cross Reactivity	No cross reactivity with IL-1b, IL-2, IL-1a, IL-10, IL-12p70, Gp130, IL-17A, IL-23, IFNg, TNFa
Components	ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
Background	IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]. Transcript Variant: This variant (3) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START#Transcript exon combination :: AK295908.1, DC336490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns ERS025083, ERS025087 [ECO:0000348] ##Evidence-Data-END#COMPLETENESS: complete on the 3' end.
Gene Symbol	IL33
Gene ID	90865
Standard Curve	Representative Standard Curve