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> High Sensitivity BDNF (Human, Rat) ELISA Kit (Plasma, Serum, CSF)

High Sensitivity BDNF (Human, Rat) ELISA Kit (Plasma, Serum, CSF)

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Cat# SK00752-01

Size : 96T

Brand : Aviscera Bioscience

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Phone : +1 850 650 7790


BDNF ELISA


 


Description Technical Specs More Information:
 
  • The Best Cited Brain-Derived Neurotrophic Factor (BDNF) Assay Kit
  • Specificity: Human or rat BDNF mature form. It is less 2% cross-reactivity with Pro BDNF (19-247) derived from HEK293 cells.
  • High Sensitivity: 1 pg/mL
  • Assay range: 3.9 ~ 250 pg/mL
  • Calibration: recombinant mature BDNF (Human, Mouse, Rat) derived from CHO.
  • Sample Type: Serum (160 or high dilution), Plasma
  • Protocol: SK00752-01
  • Aviscera Bioscience's Mature BDNF ELISA Kit had been upgraded to high sensitivity on March 18, 2022.
  • New High Sensitiivty BDNF ELISA SK00752-01A with sensitivity at 0.75 pg/mL and assay range at 1.9 ~125 pg/mL.
  • New Pro BDNF Human ELISA Kit SK00752-09 had been upgraded to high sensitivity formulation SK00752-09-HS for plasma samples on Oct. 19, 2022.
  • New High Sensitivity Pro BDNF Human ELISA Kit SK00752-05-HS available in stock.
  • New Human Pro BDNF (19-128) Specific ELISA Kit SK00752-10 Non-cross-reactivity with human mature BDNF and Pro BDNF (19-247).

Citations:
  • Arianna Sartori, Giuliana Metelli. et al. "Interferon-beta, but not Glatiramer Acetate treatment induces gender-specific increase in BDNF serum levels in relapsing-remitting multiple sclerosis female patients." Research Journal of Neurology and Neuro Disorders.: Sept-2019: Page 05-18
  • Brendan B. McAllister, Nicoline Bihelek, et al. "Brian-derived neurotrophic factor and trkb levels in mice tht lack vesicular zinc" BioRxiv (2019) doi: https://doi.org/10.1101/421453
  • Luisella Vigna, Federica Morelli, et al. Hericium erinaceus Improves Mood and Sleep Disorders in Patients Affected by Overweight or Obesity: Could Circulating Pro-BDNF and BDNF Be Potential Biomarkers. Hindawi. Volume 2019, Article ID 1861297, 12 pages.
  • Mizoguchi, Y., Yao, H., Imamura, Y. et al. "Lower brain-derived...": the Sefuri study. Sci Rep 10, 16442 (2020). https://doi.org/10.1038/s41598-020-73576-1
  • Jo D, Son Y, Yoon G, Song J, Kim OY. Role of Adiponectin and Brain Derived Neurotrophic Factor in Metabolic Regulation Involved in Adiposity and Body Fat Browning. Journal of Clinical Medicine. 2021; 10(1):56. https://doi.org/10.3390/jcm10010056
  • Carlos Berlanga-Macías. et al."Relationship between exclusive breastfeeding and brain derived neurotrophic factor in children" March 4, 2021 https://doi.org/10.1371/journal.pone.0248023
  • Lodo, Leandro, et al. and Marcelo S. "Resistance Exercise Intensity Does Not Influence Neurotrophic Factors Response in Equated Volume Schemes" Journal of Human Kinetics, vol.74, no.1, 2020, pp.227-236.
  • Aaron S. Weisbrod, et al. Decreased BDNF in female but not male rats after exposure to stress: a sex-sensitive rat model of stress? The International Journal on the Biology of Stress Volume 22, 2019 - Issue 5:581-591
  • de Menezes Galvão Ana Cecília, Raíssa Nobrega Almeida, et al. Pathophysiology of Major Depression by Clinical Stages. Frontiers in Psychology, Vol 12, 2021, 1-12, Article:641779
  • Castelli V, et al. Neuroprotective effects of human amniotic fluid stem cells-derived secretome in an ischemia/reperfusion model. Stem Cells Transl Med. 2021 Feb;10(2):251-266.
  • Thibaut Gelle, et al.BDNF and pro-BDNF in serum and exosomes in major depression: Evolution after antidepressant treatment, Progress in Neuro-Psychopharmacology and Biological Psychiatry,Volume 109,2021,
  • Serrao, J.M., Goodchild, C.S. Alfaxalone anaesthesia increases brain derived neurotrophic factor levels and preserves postoperative cognition by activating pregnane-X receptors: an in vitro study and a double blind randomised controlled trial. BMC Anesthesiol 22, 401 (2022). https://doi.org/10.1186/s12871-022-01940-x
New Research:
  • Belbin, Olivia, et al. The epistasis project: A multi-cohort study of the effects of BDNF, DBH, and SORT1 epistasis on Alzheimer's disease risk. Journal of Alzheimer's Disease 68: 1535- 1547 (2019)

  • Jo D, Song J. Irisin Acts via the PGC-1α and BDNF Pathway to Improve Depression-like Behavior. Clin Nutr Res. 2021 Oct 20;10(4):292-302. PMID: 34796134; PMCID: PMC8575642.
  • Kim J, et al. Ca2+-Permeable TRPV1 Receptor Mediates Neuroprotective Effects in a Mouse Model of Alzheimer's Disease via BDNF/CREB Signaling Pathway. Mol Cells. 2023 May 31;46(5):319-328. Epub 2023 Apr 18. PMID: 37070458; PMCID: PMC10183797.




  • (a) Pro-BDNF, BDNF, and (b) pro-BDNF/BDNF ratio serum levels ± SEM in patients treated with H. erinaceus at T0, T1, and T2. ∗p<0.05 indicates significant differences between different time versus T0 tested by paired Student's t-test. The comparison of values between T1 and T2 does not show any statistically significant differences.
    Pro BDNF ELISA Kit SK00752-09 and BDNF ELISA Kit SK00752-01 had been used by Dr.     Luisella Vigna in the research.
    Vigna, Luisella et al. “Hericium erinaceus Improves Mood and Sleep Disorders in Patients Affected by Overweight or Obesity: Could Circulating Pro-BDNF and BDNF Be Potential Biomarkers?.” Evidence-based complementary and alternative medicine : eCAM vol. 2019 7861297. 18 Apr. 2019, doi:10.1155/2019/7861297


    Rat serum BDNF was determined with SK00752-01 on this research.
    Aaron S. Weisbrod, et al. Decreased BDNF in female but not male rats after exposure to stress: a sex-sensitive rat model of stress? The International Journal on the Biology of Stress Volume 22, 2019 - Issue 5:581-591






Citations:
  • This kit had been used by Dr. Hashimoto on The Open Clinical Chemistry Journal, 2012, 5, 7-12
  • Prof. Pier Paolo Battaglini, Alessio Polacchini, Prof. Enrico Tongiorgi “Neurotrophic Factors and Other Humoral Mediators In Chronic Stress” Settore scientifico-disciplinare: Scienze Biologiche BIO/06 ANATOMIA COMPARATA E CITOLOGIA
  • Alessio Polacchini, et al. A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays. Scientific Reports volume 5, Article number: 17989 (2015)
  • Brendan B. McAllister, Nicoline Bihelek, et al. Brain-Derived Neurotrophic Factor and Trkb Levels in Mice that Lack Vesicular Zinc: Effects of Age and Sex. The University of Calgary, 2019.
  • de Menezes Galvão Ana Cecília, Raíssa Nobrega Almeida, et al. Pathophysiology of Major Depression by Clinical Stages. Frontiers in Psychology, Vol 12, 2021, 1-12, Article:641779
https://www.biorxiv.org/content/10.1101/421453v2.full









Citation:


Features
  • Size: 96 T
    Standard Range: 3.9-250 pg/ml
    Sensitivity: 1 pg/ml
    Sample Type: Human or rat serum and plasma.
    Dilution Factor: 160 or high fold dilution for serum samples
    Sample Required: 20 uL serum
    Intra-CV: 4-6%
    Inter-CV: 4-9%
    Protocol: PDF (updated on 18 March, 2022)


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