PRL ELISA Kit (Horse)

Cat# OKCA01070

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHorse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat9388-101
1:29585-100
1:49189-99
1:89390-100
ELISA Kit PrincipleThis assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PRL and Horseradish Peroxidase (HRP) conjugated PRL. The competitive inhibition reaction is launched between with HRP labeled PRL and unlabeled PRL with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PRL in the sample. The color development is stopped and the intensity of the color is measured.
ELISA Kit Range2.5 - 1,000 ng/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)9691-105
EDTA Plasma (n=4)9788-102
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level OKCA01070 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01070 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
PRL Microplate96 Wells (12 x 8 Well strips)
PRL Lyophilized Standard6 x 0.5 mL
PRL Antibody1 x 6 mL
HRP-Conjugate1 x 6 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
20X Wash Buffer1 x 15 mL
Stop Solution1 x 7 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma
Sensitivity2.5 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
  • Reconstitution & Storage Instructions
  • Western Blotting/Immunoblotting (WB/IB) Protocol
  • Immunohistochemistry (IHC) Protocol
  • Immunocytochemistry (ICC) Protocol
  • Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
  • Blocking Peptide Competition Protocol (BPCP)
  • Immunoprecipitation (IP) Protocol
  • Antibody Array (AA) Protocol
Gene SymbolPRL
Gene Full NameProlactin
Alias SymbolsGHA1
NCBI Gene Id100034034
Protein NameProlactin
Description of TargetProlactin acts primarily on the mammary gland by promoting lactation.
Uniprot IDP12420
Protein Accession #NP_001075365.1
Nucleotide Accession #NM_001081896.1