PRL ELISA Kit (Horse)
Cat# OKCA01070
Size : 96Wells
Brand : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Horse | ||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PRL and Horseradish Peroxidase (HRP) conjugated PRL. The competitive inhibition reaction is launched between with HRP labeled PRL and unlabeled PRL with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PRL in the sample. The color development is stopped and the intensity of the color is measured. | ||||||||||||||||||
ELISA Kit Range | 2.5 - 1,000 ng/mL | ||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with known low, middle and high level OKCA01070 were tested with 20 replicates across one plate. Inter-assay Precision: 3 samples with known low, middle and high level OKCA01070 were tested on three different plates with 8 replicates, respectively. Intra-Assay CV: <8% Inter-Assay CV: <10% | ||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||
Sample Type | Serum, Plasma | ||||||||||||||||||
Sensitivity | 2.5 ng/mL | ||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sanadwich ELISA | ||||||||||||||||||
Protocol Information |
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Gene Symbol | PRL |
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Gene Full Name | Prolactin |
Alias Symbols | GHA1 |
NCBI Gene Id | 100034034 |
Protein Name | Prolactin |
Description of Target | Prolactin acts primarily on the mammary gland by promoting lactation. |
Uniprot ID | P12420 |
Protein Accession # | NP_001075365.1 |
Nucleotide Accession # | NM_001081896.1 |