COR (Cortisol) BioAssay™ ELISA Kit (Equine)

Serum, plasma, tissue homogenates and other biological fluids.

For quantitative detection of COR in serum, plasma, tissue homogenates and other biological fluids.

<0.234ng/ml

Intra-Assay CV: <8%
Inter-Assay CV: <10%

0.391-25ng/ml

Equine

This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with COR. During the reaction, COR in the sample or standard competes with a fixed amount of COR on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to COR. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of COR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microtiter Strips, 1x96 wells (8x12 wells)
Standard, 2x1 vial
Sample/Standard Dilution Buffer, 1x20ml
Antibody (Biotin) (Concentrated), 1x120ul
Antibody Dilution Buffer, 1x10ml
Streptavidin (HRP) (SABC), 1x120ul
SABC Dilution Buffer, 1x10ml
TMB Substrate, 1x10ml
Stop Solution, 1x10ml
Wash Buffer, 25X, 1x30ml

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards