TGF-Beta (Transforming Growth Factor Beta) BioAssay™ ELISA Kit (Hamster)

Cat# 396481-96T

Size : 96Tests

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Applications
E

Shipping Temp
Blue Ice

Storage Temp
4°C/-20°C

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Intended Use:
For quantitative detection of TGF-beta in serum, plasma, tissue homogenates and other biological fluids.

Sensitivity:
<18.75pg/ml

Precision:
Intra-Assay CV: <8%
Inter-Assay CV: <10%

Range:
31.25-2000pg/ml

Specificity:
Hamster

Test Principle:
This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TGF-beta antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TGF-beta antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TGF-beta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TGF-beta can be calculated.

Kit Components:
Microtiter Strips, 1x96 wells (8x12 wells)
Standard, 2x1 vial
Sample/Standard Dilution Buffer, 1x20ml
Antibody (Biotin) (Concentrated), 1x120ul
Antibody Dilution Buffer, 1x10ml
Streptavidin (HRP) (SABC), 1x120ul
SABC Dilution Buffer, 1x10ml
TMB Substrate, 1x10ml
Stop Solution, 1x10ml
Wash Buffer, 25X, 1x30ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards