ADIPOQ ELISA Kit (Hamster)
Cat# OKCA00304
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Hamster | ||||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||
| ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology ADIPOQ ELISA Kit (Hamster) (OKCA00304) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for hamster ADIPOQ has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for hamster ADIPOQ is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample hamster ADIPOQ captured in well. | ||||||||||||||||||||||||
| ELISA Kit Range | 0.469 ng/mL - 30 ng/mL | ||||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay: CV% < 8% (n = 20).Inter-assay: CV% < 10% (n = 20) | ||||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||
| Sample Type | serum, plasma, tissue homogenates | ||||||||||||||||||||||||
| Sensitivity | < 0.117 ng/mL | ||||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of hamster ADIPOQ. No significant cross-reactivity or interference between hamster ADIPOQ and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between hamster ADIPOQ and all the analogues, therefore, cross reaction may still exist. | ||||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay | ||||||||||||||||||||||||
| Gene Symbol | ADIPOQ |
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| Gene Full Name | Adiponectin |
| NCBI Gene Id | 101823810 |
| Protein Name | Adiponectin |
| Uniprot ID | C9DFG2 |
| Protein Accession # | NP_001268483 |
| Nucleotide Accession # | NM_001281554 |
| Protein Size (# AA) | 244 |
| Molecular Weight | 27 kDa |