IGF2 ELISA Kit (Fish)
Cat# OKCA01046
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Fish | ||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology IGF2 ELISA Kit (Fish) (OKCA01046) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGF2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IGF2 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 62.5 - 1,000 pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with known low, middle and high level OKCA01046 were tested with 20 replicates across one plate. Inter-assay Precision: 3 samples with known low, middle and high level OKCA01046 were tested on three different plates with 8 replicates, respectively. Intra-Assay CV: <8% Inter-Assay CV: <10% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma | ||||||||||||||||||||||
| Sensitivity | 31.25 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sanadwich ELISA | ||||||||||||||||||||||
| Protocol Information |
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| Gene Symbol | IGF2 |
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| Gene Full Name | Insulin-like growth factor II |
| Alias Symbols | IGF-II |
| NCBI Gene Id | 443325 |
| Protein Name | Insulin-like growth factor II |
| Description of Target | The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. |
| Uniprot ID | P10764 |
| Protein Accession # | NP_001009311.1 |
| Nucleotide Accession # | NM_001009311.1 |