DNA Extraction Kit, FFPE Tissue, Column, BioAssay™

Cat# 299008-50T

Size : 50Tests

Brand : US Biological

Contact local distributor :

Phone : +1 850 650 7790

Shipping Temp
Blue Ice

Storage Temp

Formalin-fixed, paraffin embedded (FFPE) tissue specimens are highly valuable sources for retrospective studies of many pathologies. Nevertheless, the extraction of nucleic acids from FFPE specimens could often be challenging, as nucleic acids become cross-linked and degraded during the archiving process. Nucleic acids obtained are usually highly fragmented and chemically modified from the archiving process.

Sample Type:
FFPE tissues

Test Principle:
This FFPE Tissue DNA Extraction BioAssay™ Kit - Column allows for facile and efficient deoxyribonucleic acid extraction from FFPE tissues, with potential high throughput capabilities and full compatibility for downstream applications such as qPCR. Utilizing heat and proteinase K treatment, The FFPE Tissue DNA Extraction Kit- Column is optimized in the removal of paraffin, partial reversal of formalin cross linking, and release of DNA from fixed tissues. After clean up with DNA binding columns, the concentration of the high purity DNA can be determined by spectrophotometer or nanodrop equipment.

Kit Components:
Dewaxil, 1x28ml
Proteinase K, 1x1.1ml
FFPE Lysis Buffer, 1x10ml
Binding Buffer, 1x11ml
Wash Buffer 1, 1x bottle
Wash Buffer 2, 1x bottle
Elution Buffer, 1x2.8ml
Column, 50x columns
Actin control primer,1x tube

Quality Control:
All kit components are DNase-, RNase-, and protease-free. Each component has been tested for purity and efficacy.

Storage and Stability:
Aliquot Proteinase K into appropriate amounts and store aliquots and actin control primer at -20°C upon receipt of the kit. Store all other components at room temperature.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.