VWF ELISA Kit (Dog)
Cat# OKCD02985
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for VWF ELISA Kit (Dog) (OKCD02985) |
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| Predicted Species Reactivity | Canis lupus|Dog | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 2h | ||||||||||||||||||||||
| ELISA Kit Linearity | |||||||||||||||||||||||
| ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Von Willebrand Factor (vWF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Von Willebrand Factor (vWF) and unlabeled Von Willebrand Factor (vWF) (Standards or samples) with the pre-coated antibody specific to Von Willebrand Factor (vWF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample. | ||||||||||||||||||||||
| ELISA Kit Range | 9.88-800ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | |||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Plasma | ||||||||||||||||||||||
| Sensitivity | < 4.11 ng/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Von Willebrand Factor (vWF). No significant cross-reactivity or interference between Von Willebrand Factor (vWF) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive Inhibition ELISA |
| Gene Symbol | VWF |
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| Gene Full Name | von Willebrand factor |
| Alias Symbols | F8VWF;von Willebrand factor. |
| NCBI Gene Id | 399544 |
| Protein Name | von Willebrand factor |
| Description of Target | Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma (By similarity). |
| Uniprot ID | Q28295 |
| Protein Accession # | NP_001002932.1 |
| Nucleotide Accession # | NM_001002932.1 |
| Molecular Weight | 2813 kDa |