GAST ELISA Kit (Dog)
Cat# OKCD02581
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for GAST ELISA Kit (Dog) (OKCD02581) |
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| Predicted Species Reactivity | Canis lupus|Dog | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 2h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gastrin (GT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gastrin (GT) and unlabeled Gastrin (GT) (Standards or samples) with the pre-coated antibody specific to Gastrin (GT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gastrin (GT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gastrin (GT) in the sample. | ||||||||||||||||||||||
| ELISA Kit Range | 12.35-1,000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gastrin (GT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gastrin (GT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | < 4.95 pg/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Gastrin (GT). No significant cross-reactivity or interference between Gastrin (GT) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive Inhibition ELISA |
| Gene Symbol | GAST |
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| Gene Full Name | gastrin |
| Alias Symbols | GAS;gastrin. |
| NCBI Gene Id | 100685087 |
| Protein Name | Gastrin |
| Description of Target | Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. |
| Uniprot ID | P01353 |
| Protein Accession # | XP_003435286.1 |
| Nucleotide Accession # | XM_003435238.3 |
| Molecular Weight | 104 kDa |