FTL ELISA Kit (Dog)

Cat# OKEH03766

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FTL ELISA Kit (Dog) (OKEH03766) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FTL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FTL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FTL captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Component
ComponentAmount
Anti-FTL Microplate96 Wells (12 x 8 Well strips)
FTL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated FTL Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.059 ng/mL
SpecificityNatural and recombinant Dog Ferritin light chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFTL
Gene Full Nameferritin light chain
Alias Symbolsferritin L subunit;ferritin light chain;ferritin, light polypeptide.
NCBI Gene Id477042
Protein NameQ53VB8
Description of TargetFerritin light chain
Uniprot IDhttps://www.uniprot.org/uniprot/Q53VB8
Protein Accession #NP_001019807.1
Nucleotide Accession #NM_001024636.2
Protein Size (# AA)175
Molecular Weight20 kDa