COL1A2 ELISA Kit (Dog)

Cat# OKCD00790

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for COL1A2 ELISA Kit (Dog) (OKCD00790)
Product Info
Predicted Species ReactivityCanis lupus|Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)94-102%78-94%97-104%92-101%
EDTA plasma(n=5)96-105%79-98%83-96%82-98%
heparin plasma(n=5)88-102%78-98%83-97%90-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 2 (COL1a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 2 (COL1a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 2 (COL1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type I Alpha 2 (COL1a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)85-9689
EDTA plasma(n=5)97-105102
heparin plasma(n=5)82-10590
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-COL1A2 Microplate96 Wells (12 x 8 Well Strips)
COL1A2 Lyophilized Standard2 x 20 ng
100X Biotinylated COL1A2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.126 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 2 (COL1a2). No significant cross-reactivity or interference between Collagen Type I Alpha 2 (COL1a2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCOL1A2
Gene Full Namecollagen type I alpha 2 chain
Alias Symbolsalpha-2 type I collagen;COL1A2 gene exons 8 through 12;collagen alpha-2(I) chain;type I procollagen pro-alpha 2 chain.
NCBI Gene Id403824
Protein NameCollagen alpha-2(I) chain
Description of TargetType I collagen is a member of group I collagen (fibrillar forming collagen).
Uniprot IDO46392
Protein Accession #NP_001003187
Nucleotide Accession #NM_001003187.1
Protein Size (# AA)1366