AFP ELISA Kit (Dog)
Cat# OKCD00788
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for AFP ELISA Kit (Dog) (OKCD00788) |
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| Predicted Species Reactivity | Canis lupus|Dog | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (AFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (AFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fetoprotein (AFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-Fetoprotein (AFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Fetoprotein (AFP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Fetoprotein (AFP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids. | ||||||||||||||||||||||
| Sensitivity | < 0.115 ng/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (aFP). No significant cross-reactivity or interference between Alpha-Fetoprotein (aFP) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
| Gene Symbol | AFP |
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| Gene Full Name | alpha fetoprotein |
| Alias Symbols | alpha-1-fetoprotein;alpha-fetoglobulin;alpha-fetoprotein. |
| NCBI Gene Id | 403551 |
| Protein Name | Alpha-fetoprotein |
| Description of Target | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. |
| Uniprot ID | Q8MJU5 |
| Protein Accession # | NP_001003027 |
| Nucleotide Accession # | NM_001003027.1 |
| Protein Size (# AA) | 609 |