Taq Plus DNA Protein
Cat# OPPA00829
Size : 250U
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for OPPA00829 |
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| Host | Recombinant E.coli contains Thermus aquaticus polymerase gene |
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| Additional Information | Unit Definition: One unit of the enzyme catalyzes the incorporation of 10umole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74C. Storage Buffer: 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, Stabilizers, and 50%glycerol. |
| :: | Optimization of DNA Synthesis: It is important to ad the reaction components in the following order1. H2O.2. 10 x reaction buffer.3. dNTPs.4. DNA template and primers.5. Taq Plus. |
| :: | Product Description: Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78 degree C and remains > 95% active following 1-hour incubation at 95 degree C. |
| Reconstitution and Storage | Stable for 5 days at 10C, for longer period of time store at -20C. |
| Gene Symbol | Taq Plus DNA |
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| Alias Symbols | Taq Plus DNA Polymerase, Taq Plus DNA, TaqPDNA, Taq Plus DNA, Taq Plus DNA Polymerase Recombinant |
| Description of Target | Recombinant Taq Plus DNA Polymerase |