K1032-500 | hyPerFUsion™ high-fidelity DNA polymerase Biotrend

Product Citation

1. Qiu F, Zeng J, et al. "Functional genomics analysis reveals two novel genes required for littorine biosynthesis." New Phytol. 2019 Nov 9. PMID:31705812
2. Sun L, Büeler H. "Proteasome inhibition promotes mono-ubiquitination and nuclear translocation of mature (52 kDa) PINK1." Biochem Biophys Res Commun. 2019 Jul 27. pii: S0006-291X(19)31401-9. PMID:31362890

Description

HyperFusion™ high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, HyperFusion™ DNA polymerases produce PCR products with very high speed and accuracy. Also, HyperFusion™ DNA polymerases are tolerant of various inhibitors, allowing for robust amplification with minimal optimization. HyperFusion™ DNA Polymerase is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. HyperFusion™ high-fidelity DNA polymerases have become the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. HyperFusion™ DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50 fold lower than that of Taq DNA Polymerase and 6 fold lower than that of Pyrococcus furiosus DNA Polymerase (1), HyperFusion™ is one of the most accurate thermostable polymerases available. HyperFusion™ DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
HyperFusion™ DNA Polymerase is supplied with standard 5X HyperFusion™ Buffer, which can be used for complex or GC-rich templates. Reactions can also be optimized using the provided DMSO.

Quality Control

Protocol

Related Biological Data

HyperFusion™ high-fidelity DNA polymerase

Related Biological Data

Components and Storage

Solutions	Concentration	Storage
HyperFusion™ High-Fidelity DNA Polymerase	1,000 units/mL	-20°C
5X HyperFusion™ Buffer	5X	-20°C
Product Source	An E. coli strain that carries the hyPerFUsion™ DNA Polymerase gene.

Specialty

1. High fidelity—up to 50X more accurate than Taq; up to 6X more accurate than Pfu DNA polymerase.
2. Enhanced robustness—fewer reaction failures and minimal optimization.
3. Improved yields—high product yields with minimal enzyme amounts (0.5–1 U/50 μL reaction).
4. High speed—increased processivity allows shorter reaction times (10X faster than Pfu).
5. Versatile – Can be used for routine PCR as well as long or difficult (GC-rich) templates.

Storage

Store at -20°C.

hyPerFUsion™ high-fidelity DNA polymerase

Cat# K1032-500

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Brand : ApexBio Technology

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Product Citation

Description

Quality Control

Related Biological Data

Related Biological Data

Components and Storage

Specialty

Storage