VTG2 ELISA Kit (Chicken)

Cat# OKWB00133

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityChicken
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-100%90-104%85-105%88-104%
EDTA Plasma (n=5)85-98%83-101%88-101%82-96%
Heparin Plasma (n=5)81-89%82-89%84-100%88-98%
ELISA Kit PrincipleAviva Systems Biology Vtg2 ELISA Kit (Chicken) (OKWB00133) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Vtg2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Vtg2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Vtg2 captured in the well.
ELISA Kit Range3.125 - 200 ng/mL
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)90-9894
EDTA Plasma (n=5)86-10496
Heparin Plasma (n=5)89-10599
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Vtg2 Microplate96 Wells (12 x 8 Well strips)
Vtg2 Lyophilized Standard2 x 200 ng
100X Vtg2 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity1.875 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolVTG2
Alias Symbolsvit, VIT2, vitellogenin-2
NCBI Gene Id424533
Protein NameVitellogenin-2
Description of TargetPrecursor of the major egg-yolk proteins that are sources of nutrients during early development of oviparous organisms. Phosvitin is believed to be of importance in sequestering calcium, iron and other cations for the developing embryo.
Uniprot IDP02845
Protein Accession #NP_001026447
Nucleotide Accession #NM_001031276