TOP2B ELISA Kit (Chicken)

Cat# OKEH06630

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityChicken, Gallus gallus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Top2b ELISA Kit (Chicken) (OKEH06630) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Top2b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Top2b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Top2b captured in the well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Top2b Microplate96 Wells (12 x 8 Well strips)
Top2b Lyophilized Standard2 x 20 ng
100X Top2b HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.082 ng/mL
SpecificityNatural and recombinant Chicken DNA topoisomerase 2-beta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTOP2B
Gene Full Nametopoisomerase (DNA) II beta
Alias SymbolsDNA topoisomerase 2-beta;DNA topoisomerase II, beta isozyme;DNA topoisomerase II_beta;topoisomerase (DNA) II beta 180kDa;topoisomerase II beta-2.
NCBI Gene Id395966
Protein NameO42131
Description of TargetDNA topoisomerase 2-beta
Uniprot IDhttps://www.uniprot.org/uniprot/O42131
Protein Accession #NP_990413
Nucleotide Accession #NM_205082