IL8 ELISA Kit (Chicken)

Cat# OKEH04135

Size : 96T

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityChicken, Gallus gallus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL8 ELISA Kit (Chicken) (OKEH04135) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL8 captured in the well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.9% (n = 20)
Mean Inter-assay CV%: <= 11.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-IL8 Microplate96 Wells (12 x 8 Well Strips)
IL8 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
IL8 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity5.1 pg/mL
SpecificityNatural and recombinant Chicken Interleukin-8
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL8L2
Gene Full Nameinterleukin 8-like 2
Alias Symbols9E3;CEF-4;CEF-4/9E3 cytokine;chemokine (C-X-C motif) ligand 8;C-X-C motif chemokine 8;CXCL8;CXCLi2;embryo fibroblast protein 1;EMF-1;IL8;IL-8;interleukin-8;putatitve interleukin-8;RSV-induced protein;transformation-induced protein.
NCBI Gene Id396495
Protein NameP08317
Description of TargetInterleukin-8
Uniprot IDhttps://www.uniprot.org/uniprot/P08317
Protein Accession #NP_990829.1
Nucleotide Accession #NM_205498.1
Protein Size (# AA)103
Molecular Weight11 kDa