IFNA3 Chemi-Luminescent ELISA Kit (Chicken)

Cat# OKCD03869

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for IFNA3 Chemi-Luminescent ELISA Kit (Chicken) (OKCD03869)
Product Info
Predicted Species ReactivityChicken|Gallus gallus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)82-101%88-97%93-102%78-101%
EDTA Plasma (n=5)79-97%82-101%78-101%90-104%
Heparin Plasma (n=5)79-101%78-99%79-89%87-96%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha (IFNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha (IFNa) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)85-10194
EDTA Plasma (n=5)81-10287
Heparin Plasma (n=5)85-9791
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Ifna3 Microplate96 Wells (12 x 8 Well strips)
Ifna3 Lyophilized Standard2
100X Ifna3 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.6 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIFNA3|LOC100857744|LOC100857947|LOC100858177|LOC100859032
Gene Full Nameinterferon|interferon type A1/A2-like
Alias SymbolsIFN alpha;IFNA;IFNA1;IFNA2;IFNA6;IFNAL4;IFNAL5;IFNAL6;IFN-alpha;IFN-gamma;interferon alpha;interferon type A1/A2;interferon type A1/A2-like 4;interferon type A1/A2-like 5;interferon type A1/A2-like 6;interferon type A3.
NCBI Gene Id100857744|100857947|100858177|100859032|396398
Protein NameInterferon type A1/A2
Description of TargetHas antiviral activities.
Uniprot IDP42165
Protein Accession #NP_990758
Nucleotide Accession #NM_205427