IFNA3 Chemi-Luminescent ELISA Kit (Chicken)
Cat# OKCD03869
Size : 96Wells
Brand : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for IFNA3 Chemi-Luminescent ELISA Kit (Chicken) (OKCD03869) |
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Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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Application | Chemiluminescent immunoassay | ||||||||||||||||||||||
ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha (IFNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha (IFNa) level in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 1.37-1,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | 15.6 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | IFNA3|LOC100857744|LOC100857947|LOC100858177|LOC100859032 |
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Gene Full Name | interferon|interferon type A1/A2-like |
Alias Symbols | IFN alpha;IFNA;IFNA1;IFNA2;IFNA6;IFNAL4;IFNAL5;IFNAL6;IFN-alpha;IFN-gamma;interferon alpha;interferon type A1/A2;interferon type A1/A2-like 4;interferon type A1/A2-like 5;interferon type A1/A2-like 6;interferon type A3. |
NCBI Gene Id | 100857744|100857947|100858177|100859032|396398 |
Protein Name | Interferon type A1/A2 |
Description of Target | Has antiviral activities. |
Uniprot ID | P42165 |
Protein Accession # | NP_990758 |
Nucleotide Accession # | NM_205427 |