GCG ELISA Kit (Chicken)
Cat# OKEH06982
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Chicken, Gallus gallus | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Gcg ELISA Kit (Chicken) (OKEH06982) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gcg has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gcg is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gcg captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 31.2-2000pg/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 11.6 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Chicken Glucagon | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | GCG |
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| Gene Full Name | glucagon |
| Alias Symbols | glicentin-related polypeptide;GLP1;GLP2;glucagon preproprotein;glucagon-like peptide 1;glucagon-like peptide 2;GRPP;preproglucagon A;preproglucagon B;pro-glucagon. |
| NCBI Gene Id | 396196 |
| Protein Name | P68259 |
| Description of Target | Glucagon |
| Uniprot ID | https://www.uniprot.org/uniprot/P68259 |
| Protein Accession # | NP_001177094 |
| Nucleotide Accession # | NM_001190165 |