FASN Chemi-Luminescent ELISA Kit (Chicken)
Cat# OKCD03754
Size : 96Wells
Brand : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for FASN Chemi-Luminescent ELISA Kit (Chicken) (OKCD03754) |
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Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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Application | Chemiluminescent immunoassay | ||||||||||||||||||||||
ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Synthase (FASN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fatty Acid Synthase (FASN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fatty Acid Synthase (FASN) level in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 5.49-4,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | 2.5 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Fasn |
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Alias Symbols | FAS, Fatty acid synthase |
NCBI Gene Id | 396061 |
Protein Name | Fatty acid synthase |
Description of Target | Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities as an acyl carrier protein. |
Uniprot ID | P12276 |
Protein Accession # | NP_990486 |
Nucleotide Accession # | NM_205155 |