CAV1 ELISA Kit (Chicken)
Cat# OKCD02425
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for CAV1 ELISA kit (Chicken) |
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| Predicted Species Reactivity | Chicken | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colormetric|Regular detection | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caveolin 1 (CAV1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caveolin 1 (CAV1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caveolin 1 (CAV1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Caveolin 1 (CAV1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caveolin 1 (CAV1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caveolin 1 (CAV1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C, -20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.064 ng/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Caveolin 1 (CAV1). No significant cross-reactivity or interference between Caveolin 1 (CAV1) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
| Protocol Information |
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| Gene Symbol | CAV1 |
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| Gene Full Name | caveolin 1 |
| Alias Symbols | caveolin-1 |
| NCBI Gene Id | 373996 |
| Protein Name | Caveolin-1 |
| Description of Target | May act as a positive regulator of T-cell coactivation. May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. |
| Uniprot ID | P35431 |
| Protein Accession # | NP_001099134.1 |
| Nucleotide Accession # | NM_001105664.2 |
| Protein Size (# AA) | 178 |