BMP4 Chemi-Luminescent ELISA Kit (Chicken)

Cat# OKCD03727

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for BMP4 Chemi-Luminescent ELISA Kit (Chicken) (OKCD03727)
Product Info
Predicted Species ReactivityChicken|Gallus gallus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-101%95-105%87-101%99-105%
EDTA Plasma (n=5)87-95%83-101%91-98%93-101%
Heparin Plasma (n=5)79-104%78-103%78-99%78-92%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 4 (BMP4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 4 (BMP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 4 (BMP4) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)80-9086
EDTA Plasma (n=5)86-9490
Heparin Plasma (n=5)84-10390
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Bmp4 Microplate96 Wells (12 x 8 Well strips)
Bmp4 Lyophilized Standard2
100X Bmp4 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.156 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBmp4
Alias SymbolsBMP-4
NCBI Gene Id396165
Protein NameBone morphogenetic protein 4
Description of TargetNegatively regulates the structure and function of the limb apical ectodermal ridge.
Uniprot IDQ90752